5WJM
Crystal Structure of Mouse Cadherin-23 EC17-18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 122.476, 122.476, 43.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.600 - 2.900 |
| R-factor | 0.20326 |
| Rwork | 0.200 |
| R-free | 0.26592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wd0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.600 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.245 | 0.833 |
| Number of reflections | 7803 | 366 |
| <I/σ(I)> | 7.2 | 1.72 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 277 | 0.1 M NaOAc pH 5.2 0.01 M MgCl2 25 % (v/v) MPD 25 mM 18-Crown-6 0.07 M CaCl2 |
