5WJH
Using sound pulses to solve the crystal harvesting bottleneck
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.257, 68.257, 106.417 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.420 - 1.630 |
| R-factor | 0.19121 |
| Rwork | 0.188 |
| R-free | 0.24924 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fon |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.841 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 106.420 |
| High resolution limit [Å] | 1.630 |
| Number of reflections | 30334 |
| <I/σ(I)> | 9.4 |
| Completeness [%] | 99.7 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 295 | 0.1 M BisTris pH 6.5, 0.08M CaCl2 |
