5WJF

Crystal structure of murine 4-1BB from HEK293T cells in P21212 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	2.0
Spacegroup name	P 21 21 2
Unit cell lengths	65.050, 67.760, 87.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.900 - 2.600
R-factor	0.2282
Rwork	0.222
R-free	0.28760
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.188
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	17.900	17.900	2.550
High resolution limit [Å]	2.490	11.120	2.490
Rmerge	0.155	0.043	2.357
Rmeas	0.156	0.043	2.388
Number of reflections	26227	291	1727
<I/σ(I)>	33.04	130.91	1.5
Completeness [%]	99.1	97.7	88.5
Redundancy	70.055	76.275	39.415
CC(1/2)	1.000	1.000	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M bicine and 30% W/V PEG 6000