5WJB
Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.456, 93.706, 234.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.638 - 2.905 |
| R-factor | 0.2514 |
| Rwork | 0.249 |
| R-free | 0.29970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1no4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.583 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.108 | 0.401 |
| Number of reflections | 13797 | 688 |
| <I/σ(I)> | 19.296 | 2.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride |
