5WJ8
Crystal Structure of Human Cadherin-23 EC13-14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 74.712, 74.712, 213.507 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.170 - 1.860 |
| R-factor | 0.21843 |
| Rwork | 0.217 |
| R-free | 0.24828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vh2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.170 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.076 | 0.538 |
| Number of reflections | 30738 | 1246 |
| <I/σ(I)> | 26.8 | 2 |
| Completeness [%] | 94.4 | 83.3 |
| Redundancy | 10.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.1 M NaOAc pH 4.5 0.2 M CaCl2 30 (v/v) isopropanol 20% (v/v) glycerol |
