5WIQ

Crystal structure of the segment, GFNGGFG, from the low complexity domain of TDP-43, residues 396-402

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	4.810, 16.439, 22.672
Unit cell angles	90.63, 96.37, 91.26

Refinement procedure

Resolution	22.530 - 1.250
R-factor	0.1533
Rwork	0.152
R-free	0.16780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5WDB
RMSD bond length	0.005
RMSD bond angle	0.692
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.290
High resolution limit [Å]	1.250	2.690	1.250
Rmerge	0.161	0.069	0.406
Total number of observations	7394
Number of reflections	1802		166
<I/σ(I)>	5
Completeness [%]	94.4	99.5	95.4
Redundancy	4.1	5.5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	100mM sodium acetate pH 4.5, 800mM sodium phosphate monobasic, 1200mM potassium phosphate dibasic