5WID
Structure of a flavodoxin from the domain Archaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91810 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 112.773, 112.773, 173.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.087 - 1.681 |
| R-factor | 0.1591 |
| Rwork | 0.158 |
| R-free | 0.17330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ycf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.888 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.680 |
| Number of reflections | 73408 |
| <I/σ(I)> | 30.3 |
| Completeness [%] | 98.1 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Bis-Tris (pH 6.5), 0.2 M ammonium acetate and 20-26 % (w/v) 2-methyl-2,4-pentanediol (MPD) |
