5WHG

Vms1 mitochondrial localization core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97862
Spacegroup name	P 32 2 1
Unit cell lengths	62.864, 62.864, 154.383
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.062 - 2.700
R-factor	0.193
Rwork	0.187
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.900
High resolution limit [Å]	2.700	6.030	2.800
Rmerge	0.070	0.041	0.763
Rmeas	0.074	0.043	0.807
Rpim	0.023	0.013	0.262
Number of reflections	10237
<I/σ(I)>	9.7
Completeness [%]	96.6	99.8	66
Redundancy	10.3	10.4	9.3
CC(1/2)		0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	286	22-24% PEG MME 2000, 100 mM Tris pH 8.5, and 0.2 M TMAO