5WFY

Crystal structure of DNA-binding domain of the bacteriophage T4 ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	28.811, 40.801, 51.072
Unit cell angles	90.00, 92.35, 90.00

Refinement procedure

Resolution	51.029 - 1.400
R-factor	0.1443
Rwork	0.142
R-free	0.18150
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.030	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.095	1.346
Number of reflections	23239	3313
<I/σ(I)>	16.3	1.3
Completeness [%]	98.9	97.5
Redundancy	7.7	7.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350