5WF1
Tepsin VHS/ENTHlike domain SeMet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-10-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 65 |
| Unit cell lengths | 58.775, 58.775, 69.522 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.387 - 1.954 |
| R-factor | 0.1882 |
| Rwork | 0.184 |
| R-free | 0.22240 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.765 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rpim | 0.035 | 0.309 |
| Number of reflections | 9904 | |
| <I/σ(I)> | 28.14 | 2.25 |
| Completeness [%] | 99.0 | 99.73 |
| Redundancy | 6.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350 |
