5WDR

Choanoflagellate Salpingoeca rosetta Ras with GMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 1 21 1
Unit cell lengths	38.562, 119.106, 38.570
Unit cell angles	90.00, 117.34, 90.00

Refinement procedure

Resolution	34.261 - 1.600
R-factor	0.231
Rwork	0.229
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wds
RMSD bond length	0.011
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.700	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.098	0.944
Rpim	0.047	0.557
Number of reflections	40589	1969
<I/σ(I)>	9.5	1.4
Completeness [%]	99.9	99.8
Redundancy	4.5	3.8
CC(1/2)	0.997	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	15 % PEG8000, 100 mM Bis-Tris, pH 5.5, 200 mM magnesium acetate, 50 mM calcium chloride