5WDF
Crystal structure of 10E8v4-5R+100cF Fab in complex with HIV-1 gp41 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2017-04-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.284, 60.953, 70.149 |
| Unit cell angles | 103.08, 107.46, 100.05 |
Refinement procedure
| Resolution | 45.121 - 3.100 |
| R-factor | 0.2486 |
| Rwork | 0.247 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iq9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.605 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.111 | 0.484 |
| Number of reflections | 18005 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 93.2 | 83.6 |
| Redundancy | 2.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12% PEG3350, 10% iso-propanol, 0.1M Ammonium citrate, pH 7.5 |
