Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9176 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 187.608, 187.608, 151.363 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.079 - 1.850 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f9t |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.776 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.690 | 47.690 | 1.880 |
| High resolution limit [Å] | 1.850 | 9.790 | 1.850 |
| Rmerge | 0.048 | 0.021 | 0.992 |
| Rmeas | 0.052 | 0.024 | 1.087 |
| Rpim | 0.020 | 0.010 | 0.433 |
| Total number of observations | 549983 | 2636 | 29414 |
| Number of reflections | 86307 | ||
| <I/σ(I)> | 22.2 | 56.3 | 2 |
| Completeness [%] | 99.7 | 89.1 | 100 |
| Redundancy | 6.4 | 4.8 | 6.4 |
| CC(1/2) | 1.000 | 0.999 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1.2M sodium citrate, 0.1M HEPES |
