5WD9
Crystal structure of Legionella pneumophila effector lpg2328
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9779 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.505, 24.852, 41.297 |
| Unit cell angles | 90.00, 114.58, 90.00 |
Refinement procedure
| Resolution | 22.440 - 1.400 |
| R-factor | 0.1656 |
| Rwork | 0.164 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1000228600 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.440 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 15317 | 1522 |
| <I/σ(I)> | 19.2 | 8.8 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.5 |
