5WCU
Crystal structure of 167 bp nucleosome bound to the globular domain of linker histone H5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.926, 108.543, 180.770 |
| Unit cell angles | 100.79, 90.08, 89.94 |
Refinement procedure
| Resolution | 48.620 - 5.530 |
| R-factor | 0.195 |
| Rwork | 0.189 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qlc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.751 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.630 | 5.730 |
| High resolution limit [Å] | 5.530 | 5.530 |
| Rmerge | 0.089 | 1.706 |
| Number of reflections | 15268 | |
| <I/σ(I)> | 7.3 | 0.7 |
| Completeness [%] | 97.5 | 95.7 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 0.1 M NH4NO3, 10% MPD (v/v) |
