5WCO

Matrix Protein (M1) of Infectious Salmon Anaemia Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	38.799, 95.886, 85.410
Unit cell angles	90.00, 93.03, 90.00

Refinement procedure

Resolution	28.053 - 2.604
R-factor	0.2052
Rwork	0.203
R-free	0.25270
Structure solution method	SIRAS
RMSD bond length	0.010
RMSD bond angle	1.585
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.091	0.043	0.826
Rmeas	0.102	0.048	0.947
Rpim	0.046	0.021	0.458
Total number of observations	88204
Number of reflections	18884
<I/σ(I)>	9.4
Completeness [%]	98.5	97.6	98.2
Redundancy	4.7	4.5	4
CC(1/2)		0.998	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	293	3 uL protein solution (20 mg/mL M1 protein in 200 mM sodium chloride, 10% glycerol, 25 mM Tris-HCl, pH 7.5) + 1 uL mother liquor (0.2 M sodium chloride, 14% PEG3350, 0.1 M Bis-Tris, pH 6.5)
1	MICROBATCH	6.5	293	3 uL protein solution (20 mg/mL M1 protein in 200 mM sodium chloride, 10% glycerol, 25 mM Tris-HCl, pH 7.5) + 1 uL mother liquor (0.2 M sodium chloride, 14% PEG3350, 0.1 M Bis-Tris, pH 6.5)