5WCA
Crystal structure of the broadly neutralizing Influenza A antibody VRC 315 27-1C08 Fab.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.647, 73.780, 70.877 |
| Unit cell angles | 90.00, 93.03, 90.00 |
Refinement procedure
| Resolution | 35.389 - 1.369 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ty6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.855 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.389 |
| High resolution limit [Å] | 1.369 |
| Number of reflections | 90935 |
| <I/σ(I)> | 17.35 |
| Completeness [%] | 98.3 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 22% PEG-8000, 25% isopropanol, 0.1 M imidazole, pH 6.5 |
