5WC1
katanin AAA ATPase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-20 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 99.363, 99.363, 76.581 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.682 - 3.300 |
| R-factor | 0.2535 |
| Rwork | 0.247 |
| R-free | 0.30750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b9p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.573 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.300 |
| Rmerge | 0.098 |
| Number of reflections | 7090 |
| <I/σ(I)> | 36 |
| Completeness [%] | 98.7 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 297 | 1.0 M ammonium sulfate 0.1 M Tris pH 7.8. |
