5WBI

Crystal structure of the Arabidopsis thaliana Raptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	89.123, 112.551, 134.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.229
Rwork	0.227
R-free	0.26771
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.309
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.086	0.033	0.533
Rmeas	0.104	0.040	0.648
Rpim	0.057	0.022	0.362
Total number of observations	83089
Number of reflections	27193		2674
<I/σ(I)>	12.9
Completeness [%]	97.4	96	97.3
Redundancy	3.1	3	3
CC(1/2)		0.998	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	tacsimate