5WBF

Double CACHE (dCACHE) sensing domain of TlpC chemoreceptor from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5498
Spacegroup name	C 1 2 1
Unit cell lengths	189.330, 103.170, 61.790
Unit cell angles	90.00, 98.32, 90.00

Refinement procedure

Resolution	30.570 - 2.190
Rwork	0.182
R-free	0.21790
Structure solution method	SAD
Data reduction software	iMOSFLM
Data scaling software	Aimless (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.570	30.570	2.310
High resolution limit [Å]	2.190	6.930	2.190
Rmerge	0.062	0.037	0.280
Rmeas	0.073	0.044	0.329
Rpim	0.038	0.023	0.170
Total number of observations		6092	30803
Number of reflections	59028
<I/σ(I)>	13.3	22.3	5.3
Completeness [%]	97.6	89.9	96.3
Redundancy	3.6	3.5	3.6
CC(1/2)	0.995	0.996	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.2 M MgCl2, 0.1 M MES/NaOH (pH 6.5), 22% (w/v) PEG 4000 and 10 mM BaCl2H4O2