Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 57.580, 57.580, 183.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.940 - 1.720 |
| R-factor | 0.13601 |
| Rwork | 0.134 |
| R-free | 0.16788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qcb |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.584 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.940 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.108 | |
| Number of reflections | 16931 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.9 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 274 | 0.1 M sodium acetate, pH 4, 2.6 M ammonium sulfate |
