5WB5

Leishmania IF4E-1 bound to Leishmania 4E-IP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999909
Spacegroup name	P 62 2 2
Unit cell lengths	68.220, 68.220, 220.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.700 - 2.700
R-factor	0.2421
Rwork	0.240
R-free	0.28270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRUNCATED VERSION OF AN EIF4E M7GTP 4E-BP1 COMPLEX WITH PDB ID 1WKW
RMSD bond length	0.003
RMSD bond angle	0.484
Data reduction software	TRUNCATE
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.700	36.700	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.133	0.060	0.474
Rmeas	0.134	0.061	0.477
Total number of observations	584551
Number of reflections	9019	137	632
<I/σ(I)>	37.1	74.32	14.68
Completeness [%]	99.8	95.8	100
Redundancy	64.813	44.664	70.79
CC(1/2)	0.999	0.999	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.1 M Tris-HCl 3 % polyethylene glycol 400 2.125-2.175 M of ammonium sulfate.
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.1 M Tris-HCl 3 % polyethylene glycol 400 2.125-2.175 M of ammonium sulfate.
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.1 M Tris-HCl 3 % polyethylene glycol 400 2.125-2.175 M of ammonium sulfate.
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.1 M Tris-HCl 3 % polyethylene glycol 400 2.125-2.175 M of ammonium sulfate.
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.1 M Tris-HCl 3 % polyethylene glycol 400 2.125-2.175 M of ammonium sulfate.
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.1 M Tris-HCl 3 % polyethylene glycol 400 2.125-2.175 M of ammonium sulfate.