5WAW
FcAbVance: Increasing our knowledge of antibody structural space to enable faster and better decision-making in antibody drug discovery
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.018, 58.014, 80.442 |
| Unit cell angles | 90.00, 124.38, 90.00 |
Refinement procedure
| Resolution | 26.580 - 2.250 |
| R-factor | 0.23431 |
| Rwork | 0.231 |
| R-free | 0.27956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.336 |
| Data reduction software | HKL-2000 (0.95) |
| Data scaling software | SCALEPACK (2.2.0) |
| Phasing software | MOLREP (11.1.03) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.250 |
| Rmerge | 0.052 |
| Number of reflections | 19210 |
| <I/σ(I)> | 20 |
| Completeness [%] | 98.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1m MES pH6.5, 0.01M ZnSO4, 25% v/v PEG 550 MME |
