5WAV

Fc AbVance: Increasing our knowledge of antibody structural space to enable faster and better decision-making in antibody drug discovery.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-31
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	49.532, 79.977, 139.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.540 - 2.600
R-factor	0.2211
Rwork	0.218
R-free	0.25998
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.655
Data reduction software	HKL-2000 (0.95)
Data scaling software	SCALEPACK (0.95)
Phasing software	MOLREP (11.1.03)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.540	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.057
Number of reflections	16462
<I/σ(I)>	30
Completeness [%]	99.9
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1M Hepes pH7.5 10% v/v polyethylene glycol 8000 8% v/v ethylene glycol