5WAS

Corynebacterium glutamicum Hydrolyzed Homoserine kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	46.227, 46.227, 267.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.967 - 1.799
R-factor	0.2448
Rwork	0.241
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wat
RMSD bond length	0.006
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.799	1.800
Rmerge	0.061	0.262
Number of reflections	27709	2537
<I/σ(I)>	43.7	6.3
Completeness [%]	97.8	92.8
Redundancy	23.4	19.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	The CglThrB protein concentrated to 16.2 mg/mL in 20 mM Tris pH 7.5, 150 mM NaCl, 50 mM KCl and 50 mM MgCl2 was used for crystallization experiments. Drops were equilibrated against a 100 microLitter of well solution containing 0.25 M ammonium sulfate, 25 % PEG 3,350 and 0.1 M HEPES pH 7.5