5WAB
Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-12-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 94.610, 96.430, 106.770 |
| Unit cell angles | 87.76, 89.11, 61.00 |
Refinement procedure
| Resolution | 44.511 - 2.450 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x40 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.674 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.538 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.118 | 0.594 |
| Rpim | 0.118 | 0.594 |
| Number of reflections | 103722 | 8470 |
| <I/σ(I)> | 10.5 | 1.9 |
| Completeness [%] | 85.5 | 69.65 |
| Redundancy | 4 | 3.6 |
| CC(1/2) | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1 M HEPES pH 8.5, 0.2 M sodium chloride, 29% PEG 3350, 3% glycerol |
