5WA2

Crystal structure of Toxoplasma gondii SAG3 (SRS57)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	48.130, 59.710, 101.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.472 - 1.591
R-factor	0.1704
Rwork	0.169
R-free	0.19120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kzq
RMSD bond length	0.008
RMSD bond angle	1.275
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.472	1.620
High resolution limit [Å]	1.590	1.590
Rmerge	0.064	0.490
Number of reflections	40027	1927
<I/σ(I)>	12.6	2.8
Completeness [%]	100.0	99.8
Redundancy	5.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	25% PEG 1500