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5W8E

The structure of a CoA-dependent acyl-homoserine lactone synthase, BjaI, with the adduct of SAH and IV-CoA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]77
Detector technologyCCD
Collection date2014-02-24
DetectorRAYONIX MX-300
Wavelength(s)0.987
Spacegroup nameP 32 2 1
Unit cell lengths91.270, 91.270, 98.120
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution25.000 - 1.800
R-factor0.1966
Rwork0.196
R-free0.21709
Structure solution methodMAD
RMSD bond length0.006
RMSD bond angle1.099
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareAutoSol
Refinement softwareREFMAC (5.7.0029)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.000
High resolution limit [Å]1.8001.800
Number of reflections41676
<I/σ(I)>13.7
Completeness [%]99.2
Redundancy6.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52823.0 M sodium chloride, 0.085 M HEPES, pH 7.5, 15% v/v glycerol

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PDB entries from 2024-05-15

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