5W7O
2-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.710, 37.579, 62.319 |
| Unit cell angles | 90.00, 96.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.18445 |
| Rwork | 0.182 |
| R-free | 0.22887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8u |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.024 | 0.144 |
| Number of reflections | 18994 | 1030 |
| <I/σ(I)> | 22.5 | 3.7 |
| Completeness [%] | 89.6 | 54.2 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES pH 6.5, 12%(w/v) PEG 20000 |
