5W7I

X-ray structure of ankyrin repeat domain of DHHC17 in complex with Snap25b peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 64
Unit cell lengths	88.187, 88.187, 127.969
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.920 - 2.105
R-factor	0.1783
Rwork	0.176
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eu9
RMSD bond length	0.007
RMSD bond angle	0.817
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	29948
<I/σ(I)>	17.8
Completeness [%]	91.8
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM Tris-HCl pH 7.5, 12.5% PEG-6000