5W79
Crystal Structure of the Group II Chaperonin from Methanococcus Maripaludis, Cysteine-less mutant in the Apo State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 149.980, 149.980, 416.477 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.239 - 3.122 |
| R-factor | 0.2508 |
| Rwork | 0.249 |
| R-free | 0.28150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.850 | 3.290 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Number of reflections | 96473 | 13999 |
| <I/σ(I)> | 9.5 | 1.8 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.994 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulfate, 40% vol/vol PEG 400, 0.1 M TRIS pH 8.5 |
