5W78

Human acyloxyacyl hydrolase (AOAH), proteolytically processed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-08
Detector	RAYONIX MX-300
Wavelength(s)	1.90745
Spacegroup name	P 21 21 21
Unit cell lengths	50.402, 87.356, 146.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.661 - 2.271
R-factor	0.1676
Rwork	0.166
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.752
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.270
Number of reflections	30265
<I/σ(I)>	15.6
Completeness [%]	99.0
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	post-chymotrypsin; 100 mM sodium citrate pH 5, 20 % PEG 6000