5W6S

Crystal structure of Bacteriophage CBA120 tailspike protein 2 enzymatically active domain (TSP2dN, orf211) complex with Escherichia Coli O157-antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 43 3 2
Unit cell lengths	185.227, 185.227, 185.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.504 - 2.263
R-factor	0.1341
Rwork	0.133
R-free	0.16430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2666: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.260
Number of reflections	94879
<I/σ(I)>	26.73
Completeness [%]	99.0
Redundancy	38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	1M Lithium sulfate, 8% PEG 400, 50mM HEPES