5W6A
HLA-C*06:02 presenting ARTELYRSL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.191, 136.950, 70.961 |
| Unit cell angles | 90.00, 107.06, 90.00 |
Refinement procedure
| Resolution | 68.470 - 1.740 |
| R-factor | 0.16259 |
| Rwork | 0.161 |
| R-free | 0.20074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nt6 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.313 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.470 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rpim | 0.030 | 0.320 |
| Number of reflections | 86176 | 4252 |
| <I/σ(I)> | 18.8 | 2.7 |
| Completeness [%] | 99.6 | 92.8 |
| Redundancy | 8.7 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Bis-Tris propane pH 7.0, 0.1 M Na fluoride and 20 % PEG 8000 |
