5W69

HLA-C*06:02 presenting ARFNDLRFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	51.182, 182.137, 222.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.990 - 2.800
R-factor	0.167
Rwork	0.164
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nt6
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.340	2.890
High resolution limit [Å]	2.800	2.800
Rpim	0.110	0.510
Number of reflections	52631	4512
<I/σ(I)>	11.8
Completeness [%]	100.0	100
Redundancy	9	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris pH 8.5, 0.2 M Na acetate and 20 % PEG 3350