5W63

Crystal structure of channel catfish BAX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 2 21 21
Unit cell lengths	48.963, 58.490, 71.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.485 - 2.436
R-factor	0.1771
Rwork	0.173
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w60
RMSD bond length	0.007
RMSD bond angle	0.817
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.485	2.523
High resolution limit [Å]	2.436	2.436
Rmerge	0.152	0.619
Number of reflections	8066
<I/σ(I)>	12.86
Completeness [%]	99.0
Redundancy	6.9
CC(1/2)	0.995	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	PEG 3350, bis-TRIS, ammonium sulfate