5W62

Crystal structure of mouse BAX monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	40.787, 51.950, 62.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.236 - 2.196
R-factor	0.216
Rwork	0.208
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bd2 5w60
RMSD bond length	0.006
RMSD bond angle	0.747
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.236
High resolution limit [Å]	2.196	2.196
Rmerge	0.069	0.977
Number of reflections	7218
<I/σ(I)>	18.44
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.999	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonium sulfate, TRIS