Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-21 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.190, 114.066, 97.239 |
| Unit cell angles | 90.00, 116.85, 90.00 |
Refinement procedure
| Resolution | 35.830 - 2.650 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.754 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1156) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.060 | 0.890 |
| Number of reflections | 50632 | |
| <I/σ(I)> | 21.98 | 2.02 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.2 | 298 | 1.0 MM CAPILLARY,5 L OF 10.4 MG/ML ENZYME AND 200 L OF 100 MM MES PH 6.2, 100 MM MGCL2, AND 16% PEG 3350 |
