5W4T
Crystal Structure of Fish Cadherin-23 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 178.055, 115.548, 67.895 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 178.050 - 2.650 |
| R-factor | 0.19361 |
| Rwork | 0.192 |
| R-free | 0.22652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 178.050 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.163 | 0.685 |
| Number of reflections | 80843 | 4096 |
| <I/σ(I)> | 7.1 | 2 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 277 | 0.1 M Tris HCl pH 8.6 6% MPD |
