5W4N

Crystal structure of Streptococcus dysgalactiae SHP pheromone receptor Rgg2(C45S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-05-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	68.368, 83.268, 129.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.559 - 2.198
R-factor	0.2064
Rwork	0.205
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yv6
RMSD bond length	0.002
RMSD bond angle	0.391
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.198	5.970	2.198
Rmerge	0.073	0.044	0.546
Number of reflections	37790		1852
<I/σ(I)>	17.1		2.9
Completeness [%]	98.8	95.9	98.6
Redundancy	4.1	3.7	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	170 mM NH4OAc, 85 mM Na citrate (pH 5.6), 25.5% PEG 4,000, and 15% glycerol