5W4F

Importin binding to pol Mu NLS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	79.005, 89.819, 99.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.936 - 1.984
R-factor	0.1709
Rwork	0.170
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e6q
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.980	1.980
Rmerge	0.069	0.380
Number of reflections	48426
<I/σ(I)>	11.9
Completeness [%]	97.4
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M HEPES 0.8 M Sodium Citrate