5W4A
C. japonica N-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.770, 94.670, 72.950 |
| Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
| Resolution | 30.361 - 1.500 |
| R-factor | 0.1622 |
| Rwork | 0.162 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w4d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.989 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.361 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.081 | 1.282 |
| Rpim | 0.020 | 0.357 |
| Number of reflections | 283749 | 14301 |
| <I/σ(I)> | 16.91 | 1.85 |
| Completeness [%] | 99.8 | 99.33 |
| Redundancy | 15.1 | 13.6 |
| CC(1/2) | 0.997 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Imidazole pH 7.5, 40-45% PEG 400, 1 mM TCEP pH 7.4 |
