5W48
Crystal Structure of Riboflavin Lyase (RcaE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.970, 109.490, 126.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.480 - 1.900 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b9o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.845 |
| Data reduction software | iMOSFLM (7.2.1) |
| Data scaling software | iMOSFLM (7.2.1) |
| Phasing software | PHENIX (1.10.1-2155-000) |
| Refinement software | PHENIX (1.10.1-2155-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.480 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.200 | 2.965 |
| Number of reflections | 82558 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M Bis-Tris-HCl, 1.7 M ammonium sulfate |
