5W43

Structure of the two-component response regulator RcsB-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-11
Detector	RAYONIX MX300HE
Wavelength(s)	0.97872
Spacegroup name	P 61
Unit cell lengths	121.691, 121.691, 78.863
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.150
R-factor	0.18648
Rwork	0.183
R-free	0.25133
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5I4C & 1JE8
RMSD bond length	0.015
RMSD bond angle	2.019
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.200
High resolution limit [Å]	3.150	3.150
Rmerge	0.090	0.800
Number of reflections	11611
<I/σ(I)>	15.2	2
Completeness [%]	99.9	100
Redundancy	4.9	4.9
CC(1/2)	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.1 M Tris-HCl, 0.2 M ammonuim acetate, 25% PEG3350