5W41

Zika MR766 NLS in complex with Importin alpha subunit-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.360, 89.230, 98.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.709 - 2.200
R-factor	0.1737
Rwork	0.172
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fc8
RMSD bond length	0.009
RMSD bond angle	1.029
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.709
High resolution limit [Å]	2.200
Number of reflections	35736
<I/σ(I)>	16.9
Completeness [%]	100.0
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1M Sodium citrate, 0.01M DTT, pH6.5