5W3X
Crystal structure of PopP2 in complex with IP6, AcCoA and the WRKY domain of RRS1-R .
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.539, 87.542, 87.548 |
| Unit cell angles | 71.54, 84.04, 84.00 |
Refinement procedure
| Resolution | 82.809 - 2.000 |
| R-factor | 0.1837 |
| Rwork | 0.183 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w3t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.810 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.077 | 1.641 |
| Number of reflections | 80778 | 90528 |
| <I/σ(I)> | 11.4 | 0.5 |
| Completeness [%] | 91.8 | 63.7 |
| Redundancy | 2.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 25 mM Tris-HCl (pH 7.5), 250 mM NaCl, 50 mM Arg/Glu, 5% glycerol and 2 mM IP6 |
