5W2F
Crystal Structure of the C-terminal Domain of Human eIF2D at 1.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.650, 50.650, 183.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.830 - 1.400 |
| R-factor | 0.1419 |
| Rwork | 0.140 |
| R-free | 0.17180 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.346 |
| Data reduction software | SHELX |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.830 | 48.830 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.660 | 1.400 |
| Rmerge | 0.043 | 0.031 | 1.098 |
| Rmeas | 0.049 | 0.036 | 1.297 |
| Rpim | 0.023 | 0.017 | 0.674 |
| Total number of observations | 198682 | 1405 | 6716 |
| Number of reflections | 47791 | 354 | 2157 |
| <I/σ(I)> | 14.7 | 40.6 | 0.9 |
| Completeness [%] | 99.1 | 96.1 | 94.2 |
| Redundancy | 4.2 | 4 | 3.1 |
| CC(1/2) | 0.999 | 0.997 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 3.5 M Sodium Formate, 100 mM Sodium Acetate pH4.5 |
