5W24
Crystal Structure of 5C4 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2014-03-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.960, 63.010, 56.540 |
| Unit cell angles | 90.00, 98.60, 90.00 |
Refinement procedure
| Resolution | 32.883 - 1.500 |
| R-factor | 0.1714 |
| Rwork | 0.170 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t3p 4gag |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data reduction software | Aimless |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.883 | 37.490 | 1.530 |
| High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
| Rmerge | 0.044 | 0.035 | 0.234 |
| Rmeas | 0.051 | 0.043 | 0.275 |
| Rpim | 0.026 | 0.023 | 0.143 |
| Total number of observations | 226910 | 987 | 7853 |
| Number of reflections | 60748 | ||
| <I/σ(I)> | 14.9 | 25.6 | 3.8 |
| Completeness [%] | 93.7 | 77.1 | 69.7 |
| Redundancy | 3.7 | 3.1 | 3.5 |
| CC(1/2) | 0.998 | 0.995 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 10.1 M sodium acetate trihydrate pH 4.6 and 20% PEG 3350 |
