5W1W

Structure of the HLA-E-VMAPRTLVL/GF4 TCR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	73.371, 225.926, 276.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.290 - 3.100
R-factor	0.217
Rwork	0.215
R-free	0.26300
RMSD bond length	0.007
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.290	3.211
High resolution limit [Å]	3.100	3.100
Rmerge	0.061	0.337
Number of reflections	84282	8335
<I/σ(I)>	10.74	2.06
Completeness [%]	100.0	100
Redundancy	2	2
CC(1/2)	0.994	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 m Na.K tartrate, 20% PEG 3350