5W0X

Crystal structure of mouse TOR signaling pathway regulator-like (TIPRL) delta 94-103

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 63
Unit cell lengths	63.642, 63.642, 102.053
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.495 - 2.717
R-factor	0.2111
Rwork	0.208
R-free	0.27320
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.185
Phasing software	CRANK2 (1.9.95)
Refinement software	PHENIX ((dev_2621: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.495	2.780
High resolution limit [Å]	2.717	2.717
Rmerge	0.128	0.692
Rpim	0.041	0.266
Number of reflections	12335	296
<I/σ(I)>	31.94	1.39
Completeness [%]	97.3	45.9
Redundancy	10.5	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M Cacodylate pH6.5 15% PEG1000(v/v) 0.2M Magnesium chloride